Abstract
BackgroundCurcumin is a major constituent of rhizomes of Curcuma longa that elicits beneficial effects for oxidative damage. The aim of this study was to investigate whether curcumin could attenuate hydrogen peroxide (H2O2)-induced apoptosis in H9c2 cardiomyoblasts and the underlying mechanisms.ResultsThe present study showed that exposure of H9c2 cells to H2O2 caused a significant increase in apoptosis as evaluated by flow cytometry analysis and the pretreatment of curcumin protected against H2O2-induced apoptosis. Exposure of cells with curcumin caused a dose-dependent induction of heme oxygenase-1 (HO-1) protein expression. Curcumin also decreased the cleaved caspase-3 (CC3) protein expression level and increased the Bcl-2/Bax ratio in H2O2-stimulated H9c2 cells. ZnPP-IX, a HO-1 inhibitor, partly reversed the anti-apoptotic effect of curcumin. Further, LY294002, an inhibitor of PI3K, partially reversed the effect of curcumin on HO-1 protein induction, leading to the attenuation of curcumin-mediated apoptosis resistance.ConclusionThese results demonstrated that the anti-apoptotic function of curcumin required the upregulation of HO-1 protein through the PI3K/Akt signaling pathway. Curcumin might be used as a preventive and therapeutic agent for treatment of cardiovascular diseases associated with oxidative stress.
Highlights
Curcumin is a major constituent of rhizomes of Curcuma longa that elicits beneficial effects for oxidative damage
Our results indicate that curcumin may have protective role against hydrogen peroxide (H2O2)-induced cell death
We showed that H9c2 cells subjected to H 2O2 had decreased Bcl-2/Bax ratio compared with the control group, while curcumin pretreatment increased the Bcl-2/Bax ratio compared with the H 2O2 group
Summary
Curcumin is a major constituent of rhizomes of Curcuma longa that elicits beneficial effects for oxidative damage. The aim of this study was to investigate whether curcumin could attenuate hydrogen peroxide (H2O2)-induced apoptosis in H9c2 cardiomyoblasts and the underlying mechanisms. A major component of turmeric powder extracted from the rhizomes of the plant Curcuma longa, has been applied for centuries in indigenous medicine to treat various diseases [5]. This bioactive phytochemical is a potent inhibitor of tumor promotion and possesses antiinflammatory and anti-oxidative activities [6]. The possible protective effect of curcumin on the toxicity in cardiomyoblasts has not
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