Abstract
Ammonia-oxidising bacteria (AOB) consortium has attracted attention considering the cost savings in wastewater treatment. The study aims to optimise the composition of the AOB consortium for ammonia removal. AOB bacteria were isolated from the activated sludge of a coffee effluent bioreactor. All isolates were cultivated in a mineral salt medium containing carbon and ammonia sources in the screening test. The three effective isolates denoted as NS2, NB1 and PS were identified as Burkholderia latens (MW485434), Paenibacillus sp. (MW485433), and Providencia rettgeri (MW485435) respectively. The results showed that isolate NS2 (88.4%), NB1 (62.7%), and PS (63.3%) produced the highest ammonia removal among other isolates of their distinctive agar media. The composition of the AOB consortium (NS2, NB1, and PS) were optimised using Simplex Lattice Mixture Design. The optimised consortium ratio of these effective isolates achieved 85% and 78% of ammonia and COD removal efficiency respectively. This consortium has a great potential as a bioremediation agent for ammonia removal in wastewater reclamation.
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