Unveiling Cellular Microenvironments with a Near-Infrared Fluorescent Sensor: A Dual-Edge Tool for Cancer Detection and Drug Screening.

Abstract

Mitochondria and lysosomes are pivotal intracellular organelles, and the injury or dysfunction of these organelles can trigger a range of pathological processes. Early diagnosis and treatment of cancer are of paramount importance due to cancer's status as a leading health threat. This study introduces a novel fluorescent probe, BDHV, for detecting mitochondrial and lysosomal viscosity and pH abnormalities in tumors, facilitating early cancer detection and screening of anticancer drugs. Under acidic conditions, the red fluorescence of the probe gradually increases with increasing viscosity. Conversely, in alkaline environments, an increase in viscosity leads to a decrease in green fluorescence and an increase in red fluorescence. The inclusion of a benzothiazole group endows BDHV with strong dual-targeting capability for mitochondria and lysosomes and without being affected by the mitochondrial membrane potential. Most notably, BDHV has potential applications for early cancer diagnosis and in effectively assessing the efficacy of various anticancer drugs.