Analysis of Plasmodium falciparum growth in culture using acridine orange and flow cytometry.

Abstract

The growth of Plasmodium falciparum in cultures of human red blood cells was studied using acridine orange to stain RNA and DNA, followed by flow cytometric analysis. The cycle of the parasite is characterized by a period of growth, prior to initiation of DNA synthesis, in which a significant increase in red fluorescence is observed, with only a small change in green fluorescence. Following this phase, which is formally similar to the G1 period in mammalian cells, initiation of DNA synthesis is characterized by increases in green fluorescence. Sorting of cells from several regions of the two-dimensional display shows that the distribution of morphological stages correlates with differences in red and green fluorescence. The effect of aphidicolin on the growth cycle of the parasite was also studied.