Abstract
PurposeLovastatin is an important medicine and it shows a significant effect against glucocorticoid-induced necrosis of the femoral head. This study aimed to investigate the effect of lovastatin on preventing necrosis of the femoral head of by serum metabolomics strategy.MethodsAdult healthy adult Japanese white rabbits were divided into three groups: control group, model group, and drug group. The pathologic changes of femoral head were assessed with magnetic resonance imaging and microscope. Metabolomics based on ultra-high performance liquid chromatography tandem mass spectrometry analysis was used to analyze the collected serum sample. Data were analyzed using principal component analysis, partial least squares-discriminate analysis, and orthogonal partial least squares-discriminant analysis. All potential metabolites were identified by comparing with human metabolome database, Metlin database, lipid maps, and chemspider database.ResultsEleven potential biomarkers were noted and identified as potential biomarkers. The change of biomarkers suggested that lovastatin on preventing necrosis of the femoral head may affect glycerophospholipid metabolism, linoleic acid metabolism, sphingolipid metabolism, alpha-linolenic acid metabolism, pyrimidine metabolism, and arachidonic acid metabolism.ConclusionThe study suggested that lovastatin could prevent the glucocorticoid-induced necrosis of the femoral head of rabbits. The possible reasons were closely associated with adjusting the lipid metabolism, inhibiting adipogenesis, and delaying the osteocyte apoptosis.
Highlights
Glucocorticoid-induced necrosis of the femoral head is difficult miscellaneous diseases in clinical diagnose [1]
Article focus This study aimed to investigate the effect of lovastatin on preventing necrosis of the femoral head of by serum metabolomics strategy
Key messages Metabolomics based on ultra-high performance liquid chromatography tandem mass spectrometry analysis (UHPLC-MS/MS) was used to analyze the collected serum sample
Summary
Glucocorticoid-induced necrosis of the femoral head is difficult miscellaneous diseases in clinical diagnose [1]. The glucocorticoid is one of the steroid and plays an important role on clinical treatment of organ grafting, systemic lupus erythematosus, rheumatic arthritis, dermatomyositis, and myasthenia, but long time or excess use of the glucocorticoid could result in escalation of osteonecrosis [2,3,4,5]. The effect of glucocorticoid for disease treatment is remarkable. Because of the side effect, it is restrictive for using of glucocorticoid, resulting in influencing the treatment of diseases which need chronic or excessive use glucocorticoid [6, 7]. The effective treatment of steroid-induced avascular necrosis of the femoral head (SANFH) was appealing
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