Abstract
Objective: The present study aimed to investigate the effect of the transplantation of basic fibroblast growth factor (FGF-2) gene-transfected mesenchymal stem cells (MSCs) and xenogeneic antigen-cancellous bone (XACB) on tumor necrosis factor-α (TNF-α) expression with avascular necrosis of the femoral head (ANFH) in rabbits. Methods: The models of steroid-induced osteonecrosis in rabbits were randomly divided into five groups: A (model), B (XACB), C (XACB + MSCs), D (XACB + MSCs + LV), and E (XACB + MSCs + LV-FGF-2) groups. The therapeutic effect was evaluated by Hematoxylin and Eosin (H&E) staining. Immunohistochemical and RT-PCR assays were used to detect the protein and mRNA expression of TNF-α in the femoral head, respectively. Results: At 12 weeks after the operation, the defect in rabbits in group E was completely repaired, while defects in rabbits in the other groups were not completely repaired, and the area of new bone formation was higher, when compared with the other groups (P<0.05). Furthermore, the protein and mRNA expression TNF-α was lower at 3, 6, and 12 weeks after surgery, when compared with the other groups, and the difference was statistically significant (P<0.05). Conclusion: FGF-2/MSCs/XACB could promote the repair of ANFH, and may be correlated to the inhibition of TNF-α expression.
Highlights
Avascular necrosis of the femoral head (ANFH) is a hip joint disease characterized by severe shortage of blood supply and intraosseous pressure [1]
Glucocorticoid-induced ANFH was used as the model, and its histopathology was characterized by the necrosis of bone marrow cells and the proliferation of adipocytes in the bone marrow, which is consistent with femoral head necrosis pathological changes, and suitable for the treatment of femoral head necrosis
mesenchymal stem cell (MSC) have been used as seed cells, and the combination of gene transfection and tissue engineering has been the hotspot in the study of femoral head necrosis
Summary
Avascular necrosis of the femoral head (ANFH) is a hip joint disease characterized by severe shortage of blood supply and intraosseous pressure [1]. The incidence of femoral head necrosis has been increasing annually, which has exceeded hip joint tuberculosis and become the first in hip joint diseases [2]. There are many ways to treat femoral head necrosis, such as core decompression, bone grafting, joint formation, and joint replacement [3], and each method has its own shortcomings. Bone marrow stem cells, which are modified or enhanced by gene transfection technology, have become a new and popular treatment plan for the treatment of femoral head necrosis. In the previous experiments conducted by the investigators, basic fibroblast growth factor-2 (FGF-2) gene-transfected mesenchymal stem cells (MSCs) were used to construct tissue-engineered bone to repair the ANFH, which achieved certain results. The present study used gene transfection technology to transfect the FGF-2 gene into rabbit MSCs through the lentiviral vector and cultured this with xenogeneic antigen-cancellous bone (XACB) to produce tissue engineering bone
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