Unraveling the Importance of Forest Structure and Composition Driving Soil Microbial and Enzymatic Responses in the Subtropical Forest Soils

Abstract

As the responsive soil properties, soil microbial fractions and enzymatic activities are often recommended for assessing soil environment. Different flora, silvicultural practices, and anthropogenic activities regulate essential ecosystem processes. They could substantially affect biological properties, nutrient budgets, and biogeochemical cycles at local and regional scales. This study examined how different forest compositions influenced by various anthropogenic activities (land use change, over-exploitation, species translocation) affect soil microbial properties and enzymatic activities, as well as the effects of soil chemical properties on these patterns in important sub-tropical forest ecosystems in Southern China. The research was conducted at Lutou forest research station, located in Yueyang, Hunan Province, China. Soil samples were collected at 0–10, 10–20, and 20–40 cm depths from natural broadleaved forest (NBF), coniferous monoculture plantations (CPF), and mixed forest stand. CPF stands are directly affected by human interference and frequent harvesting practices, whereas mixed forest and NBF stands are naturally grown forests with minimal human interference. Enzymes continually play a positive role in preserving soil health. The results showed that the interaction effect of forest type and soil depth significantly influenced urease, sucrase, and protease activity (all p < 0.001); however, no clear patterns were observed. Soil microbial carbon (MBC) and soil microbial nitrogen (MBN) were remarkably higher in 0–10 cm in mixed forest and NBF stand compared to CPF stand. For the upper soil layer, soil organic carbon (SOC) was higher in mixed forest, whereas, for the remaining two layers, it was observed to be highest in NBF. Moreover, the microbial quotient (MBC/SOC) was considerably higher in NBF forest in all soil layers than in mixed forest and CPF stand. Soil organic carbon (SOC) and soil total nitrogen (TN) had a strong positive relationship with MBC compared to MBN. Our study contributes toward an enhanced understanding of soil enzymatic responses and microbial soil dynamics’ biological patterns, controls, and activities in different rural forest ecosystems.