Abstract
Clonostachys rosea is recognized for its potential as a biocontrol agent against soil-borne pathogens, yet its antifungal mechanisms remain underexplored. In the present study, five strains of Clonostachys rosea were isolated from rhizhosphere soil samples from various agroecosystem. They were and characterized morphologically using taxonomical keys, Scanning Electron Microscopy (SEM) and molecularly using Internal Transcribed Spacer (ITS) primers. The antagonistic activity of C. rosea against Macrophomina phaseolina (Mp) was evaluated through a dual culture assay. Additionally, bioactive compounds were identified using GC-MS analysis and their antifungal potential was analyzed using molecular docking simulations to predict interactions with M. phaseolina. In silico docking studies identified key bioactive compounds viz., (5S, 8R, 9S, 10S, 13S, 14S, 17S)-17-hydroxy-10,13-dimethyl-1, 2, 4, 5, 6, 7, 8, 9, 11, 12, 14, 15, 16, 17 tetradecahydrocyclopenta[a]phenanthren-3-one and 2-oxochromene-6-carbaldehyde with high binding affinity to fungal targets with low binding energy, suggesting their role in the observed antifungal activity.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
