Unprogrammierte DNA-Synthese (DNA-Reparatur) in Milz-und Thymuszellen der Ratte nach Einwirkung von UV-Licht, Röntgenstrahlen oder Methylmethansulfonat in vitro / Unscheduled DNA Synthesis (DNA-Repair) within Splenic and Thymic Cells of the Rat under the Influence of UV-Light, X-Irradiation and/or Methyl-methanesulfonate

Abstract

Unscheduled DNA synthesis (UDS) of splenic and thymic cells of the rat has been stimulated in vitro by UV-light (8-128 J x m-2), X-rays (120-3480 rd), methyl-methanesulfonate (MMS), and/or a combination of UV-light and X-irradiation. The height of UDS-induced stimulation of incorporation of [3H]thymidine into splenic and thymic cell DNA at saturation doses of UV-light (splenic cells: 8, thymic cells: 96 J x m-2) or X-irradiation (splenic cells: 960, thymic cells: approximately 3480 rd) suggest that the greater sensitivity of T-cells (represented by thymic cells) towards UV-light and the greater sensitivity of B-cells (represented by splenic cells) towards X-rays can be explained-at least partly-in terms of less efficient excision repair systems.