Abstract
The biotechnological and functional food applications of xylooligosaccharides (XOS) are determined by their degree of polymerisation (DP) and their substituents' characteristics. Separation of XOS presents a formidable challenge for their large-scale production due to their complexity and similarity. This study employs nanofiltration membranes to fractionate beechwood xylan hydrolysates containing XOS. Tubular ceramic membranes with molecular weight cut-offs of 750, 450, and 200 Da effectively separated XOS into three distinct DP ranges, namely X2-X5, X2-X4, and X2-X3. The impacts of operating pressure and pH were evaluated to find the conditions leading to high permeate flux while maintaining high selectivity. Operating pressure of 10 bar with pH 7.0 for the 750 and 450 Da membranes and pH 4.2 for the 200 Da membrane yielded purities of 85%, 71%, and 74% for X2-X5, X2-X4, and X2-X3, respectively.
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