Abstract
To enhance the production of short-chain fatty acids (SCFAs) from waste activated sludge (WAS), we introduced an alginate-degrading microbial consortium (ADC) and investigated its impact across different dosage levels on anaerobic WAS fermentation. At ADC dosages of 5%, 10%, and 20% (v/v), the soluble chemical oxygen demand (SCOD) content increased by 0.99-fold, 1.19-fold, and 1.78-fold, respectively, compared to the control. Concurrently, there was a substantial rise in the acetate ratio from 34.11% to 63.70%− 66.6%. Following ADC supplementation, the activities of protease and α-glucosidase were significantly enhanced, and hydrophobicity markedly reduced, facilitating the release of organic compounds from WAS. Microbial analysis pinpointed Tetrasphaera, Tessaracoccus, Brooklawnia, and Bacteroidetes_vadinHA17 as primary contributors to the hydrolysis of polysaccharide fraction. Metabolic analysis unveiled an overexpression of functional metabolisms in the ADC groups, which promoted the increased utilization of proteins, amino acids, and other macromolecular organics, thereby stimulating ammonia emission and SCFAs production.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
