Abstract
Distinguishing between the enantiomers of chiral substances and their quantification is an analytical challenge, in particular in the pharmaceutical and biochemical sectors. A Raman spectroscopic method for discrimination of enantiomers is proposed. Advantage is taken of the polarization properties when Raman scattering occurs in an optically active medium. It is shown that a conventional polarization-resolved Raman setup leads to identical spectra of the two enantiomers. However, inserting a half-wave retarder to rotate the signal polarization by a fixed angle enables the efficient and universal enantiomeric discrimination. Hence, the applicability of any polarization-resolved Raman experiment can be improved substantially without significant modification of the setup or the use of chiral labeling or the addition of a substrate for selective plasmonic enhancement. In principle, the proposed technique allows simultaneous speciation, enantiomeric discrimination, as well as structural and quantitative analysis.
Highlights
Distinguishing between the enantiomers of chiral substances and their quantification is an analytical challenge, in particular in the pharmaceutical and biochemical sectors
Advantage is taken of the polarization properties when Raman scattering occurs in an optically active medium
It is shown that a conventional polarization-resolved Raman setup leads to identical spectra of the two enantiomers
Summary
Open Access Article. Published on 28 January 2015. Downloaded on 10/5/2020 8:21:05 PM. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Cite this: Analyst, 2015, 140, 1787 Received 3rd December 2014, Accepted 28th January 2015 DOI: 10.1039/c4an02218a www.rsc.org/analyst
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