Abstract
The β3 subunit of αIIbβ3 and αvβ3 integrins contains four epidermal growth factor (EGF)-like domains. Each domain harbors four disulfide bonds of which one is unique for integrins. We previously discerned a regulatory role of the EGF-4 Cys-560-Cys-583 unique bond for αIIbβ3 activation. In this study we further investigated the role of all four integrin unique bonds in both αIIbβ3 and αvβ3. We created β3 mutants harboring serine substitutions of each or both cysteines that disrupt the four unique bonds (Cys-437-Cys-457 in EGF-1, Cys-473-Cys-503 in EGF-2, Cys-523-Cys-544 in EGF-3, and Cys-560-Cys-583 in EGF-4) and transfected them into baby hamster kidney cells together with normal αv or αIIb. Flow cytometry was used to measure surface expression of αIIbβ3 and αvβ3 and their activity state by soluble fibrinogen binding. Most cysteine substitutions caused similarly reduced surface expression of both receptors. Disrupting all four unique disulfide bonds by single cysteine substitutions resulted in variable constitutive activation of αIIbβ3 and αvβ3. In contrast, whereas double C437S/C457S and C473S/C503S mutations yielded constitutively active αIIbβ3 and αvβ3, the C560S/C583S mutation did not, and the C523S/C544S mutation only yielded constitutively active αIIbβ3. Activation of C523S/C544S αvβ3 mutant by activating antibody and dithiothreitol was also impaired. Molecular dynamics of C523S/C544S β3 in αIIbβ3 but not in αvβ3 displayed an altered stable conformation. Our findings indicate that unique disulfide bonds in β3 differently affect the function of αIIbβ3 and αvβ3 and suggest a free sulfhydryl-dependent regulatory role for Cys-560-Cys-583 in both αIIbβ3 and αvβ3 and for Cys-523-Cys-544 only in αvβ3.
Highlights
Disulfide bonds in 3 are involved in ␣IIb3 activation
For this purpose we created 3 mutants in which each of the four unique disulfide bonds in the four epidermal growth factor (EGF)-like domains was disrupted by cysteine to serine substitutions
Because free sulfhydryls were shown to play a role in ␣IIb3 activation by disulfide bond rearrangement [37– 40, 42], we compared the effect of substituting both cysteines to the effect of substituting only one cysteine yielding free sulfhydryls in their cysteine partners
Summary
Results: Disruptions of unique disulfide bonds in EGF domains of 3 yielded constitutively active ␣IIb3 and ␣v3 variably dependent on the presence of free sulfhydryls. We created 3 mutants harboring serine substitutions of each or both cysteines that disrupt the four unique bonds (Cys-437–Cys-457 in EGF-1, Cys-473–Cys503 in EGF-2, Cys-523–Cys-544 in EGF-3, and Cys-560 –Cys583 in EGF-4) and transfected them into baby hamster kidney cells together with normal ␣v or ␣IIb. Flow cytometry was used to measure surface expression of ␣IIb3 and ␣v3 and their activity state by soluble fibrinogen binding. Disrupting all four unique disulfide bonds by single cysteine substitutions resulted in variable constitutive activation of ␣IIb3 and ␣v3. Our findings indicate that unique disulfide bonds in 3 differently affect the function of ␣IIb3 and ␣v3 and suggest a free sulfhydryl-dependent regulatory role for Cys-560 –Cys-583 in both ␣IIb3 and ␣v3 and for Cys-523–Cys-544 only in ␣v3
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