Abstract
The utility of the UNIFAC activity coefficient determination method for understanding the magnitude of solute-solvent interactions in reversed-phase liquid chromatography (RPLC) is discussed. UNIFAC-computed partition coefficients for the transfer of various homologous series of solutes from aqueous mixtures of methanol, acetonitrile and tetrahydrofuran to hexadecane, octane, butane and benzene were used to investigate the effects of the carbon number, the type of functional group of the solutes, the composition of the mobile phases, the chain length of alkyl-bonded stationary phases and temperature on retention in reversed-phase liquid-liquid partition chromatography. Although UNIFAC is not accurate enough to be useful for the quantitative determination of retention in RPLC, it is useful in explaining a wide variety of issues of general importance in RPLC.
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