Abstract
Retention in reversed-phase liquid chromatography has been found to be related to the Van der Waals volume, pi-energy and hydrogen-bonding energy effects. However, higher-molecular-weight compounds were retained more strongly than expected. In order to investigate this effect more fully, the retention times of phenols were measured on an octadecyl-bonded silica gel in acidic acetonitrile—water mixtures at different temperatures. The enthalpies of phenols were then calculated from their log k′ values. The magnitude of the enthalpy effect increases with increasing molecular size, but the polarity of the molecule is the predominant factor in the enthalpy effect.
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