Abstract
Abstract The objective of this work was to investigate the anti-proliferation, apoptotic and antioxidant effects of unfractionated polyphenols of green tea (GRTP) and ginger (GNRP), compared to cisplatin and ascorbic acid in human hepatocellular carcinoma. The cells’ growth rate and survival (MMT assay) were investigated in cells treated with different doses of polyphenols. The apoptotic fraction of treated cells was measured by flow cytometry, whereas the antioxidant effect of polyphenols was investigated in the presence or absence of thioacetamide (TAA), and compared to that of ascorbic acid. The results indicated that GRTP and GNRP, inhibited the growth rate of cells. GRTP was more cytotoxic, where cell viability of GRTP-treated cells was lower than cells treated with GNRP. The IC 50 values were 39.3 μg/ml and 64.9 μg/ml, respectively. GRTP induced more apoptosis than GNRP or cisplatin. Moreover, the oxidative stress induced by TAA was challenged by GRTP, where reduced glutatione (GSH) in cells dually treated with GRTP/TAA was higher than untreated, GNRP/TAA and TAA/ascorbic acid treated cells. The data revealed the antihepatocarcinogenic effect of these polyphenols, where they triggered apoptosis and showed antioxidant effect in TAA treated cells. Green tea polyphenol was more antineoplasitic, apoptotic, compared to ginger polyphenols, and more antioxidant than ascorbic acid.
Published Version
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