Understanding Rapid Transmitting, Invasive, Corona Pandemics through (Aair), (IS::Tn::IS), Crispr/Cas-9 DNA Editing

Abstract

Antiadherent Immune Response (AAIR) against serotype 026: EPEC (Enteropathogenic (invasive) Escherichia coli) a fatal diarrhoea causing E.coli was successful in Balb/c mice experiment by the author. IS(Insertion Sequence) represented by IS1, IS2, IS3,-..IS10 flanking transposons (Tn) “IS::Tn::IS” and its illegitimate recombination was also studied curiously by the author to observe their spontaneous jumping, illegitimate recombination activities among DNA, chromosome and plasmids in Escherichia coli. Considering the fatality rate of corona as pandemic globally, the author has attempted to realise the possible application of AAIR, IS::Tn::IS and Crispr/Cas-9 in designing vaccine against corona, to prevent corona virus not to adhere in trachea and lung cells, to repair and to bring healthy life to mankind. is-Tn-is was observed/studied/discovered in Maize by Barbara Mc Clintock, USA, Peter Starlinger and Heinz Saedler, Germany studied the same in bacteria. Due to spontaneous mutations in corona, it is speculated by the author, that “IS::Tn::IS” DNA sequences might be present in corona, to mutate and to change the adhering, invasive and infective spike protein. The presence of IS-Tn-IS sequence have not been studied in SARS-COV-2, COVID-19, and corona. The sequence length, varied from IS1 (0.8kb) to IS10, (1.2 kb) (kilo base pairs) showed the potentiality spontaneous to do illegitimate recombination. If any of the sequence IS1, IS2, IS3…IS10 found common among HIV, MERS, SARS, Influenza, and H1N1 viruses, it could be useful to develop AAIR. Both AAIR vaccine concept and recent revolution in DNA editing, Crispr/cas-9(Clustered, Repeat Interspaced Short Palindromic Repeat), / (Crispr-associated protein-9) both could essentially be used, bidirectional in corona vaccine to protect pandemics and their repeats in future. The IR mechanisms which is proposed, that IS::Tn::IS cloned spike pathogenic DNA as involved in AAIR monoclonal antibody will block the adherence of corona spikes and Crispr/cas-9 will repair, the transmitted, invasive corona, in human body by programmable DNA of non-pathogenic influenza virus. The author describes few of those possible mechanisms of Is:: spike protein and Crispr based transposons to mobilize IR vaccine against corona, considering the nanostructures of corona and the use of it huge surface area in immune surveillance.