Understanding photochemical adaptations of microalgal ammonium extremophiles to elevated ammonium concentrations in abattoir anaerobic digestate

Abstract

Identifying microalgal species with outstanding environmental resistance to NH4+ toxicity is critical to developing effective algal-based anaerobic effluent treatment. This is the case for two microalgal NH4+ extremophiles, Chlorella sp. MUR269 and Scenedesmus sp. MUR270 well suited to treat high nitrogen digestates. However, their exact tolerance mechanisms to high NH4+ conditions still need to be understood. A chlorophyll fluorescence approach based on high-throughput pulse amplitude-modulated fluorimetry was used to understand the photochemical adaptations of these species under increasing NH4+ concentrations (0, 100, 200 mgNL−1) derived from abattoir anaerobic digestate. Both species tolerated 200 mgN-NH4+L−1 in the digestate by exhibiting different photochemical mechanisms. Chlorella decreased its PSII primary photochemistry efficiency (Fq′/FM′) and maximum electron transport (rETRmax) immediately after exposure to the treatment conditions. In contrast, Scenedesmus maintained unaltered Fq′/FM′ regardless of NH4+-N concentration, while rETRmax was significantly reduced to a level of Chlorella at 200 mgNL−1. Energy transduction parameters showed Chlorella increased the size of its reaction centre antennae to capture more photons, which increased the trapping flux ratio and maintained efficient dissipation of excess energy. Conversely, Scenedesmus displayed reductions in the absorbed, trapped and dissipated energy fluxes, though its transported flux for photochemical energy production was similar to Chlorella sp. The energetic communication and connectivity between Chlorella PSII units went up by 47 % and 40 % when the NH4+-N level increased from 50 to 100 and N200 mgL−1, respectively, while Scenedesmus exhibited a dose-dependent decrease in the connectivity between its PSII components. The photosynthetic performance index revealed that the overall energetic flux processing efficiency by Scenedesmus was 86 % (50 mgNL−1), 73 % (100 mgNL−1) and 91 % (200 mgNL−1) higher than Chlorella sp. Thus, the two chlorophytes maintained their overall photochemical capacity by simple, economic biological mechanisms involving adjustments of antenna size and energetic communications between PSII neighbour units.