Uncovering the impact of sea mud-containing diet on fecal fragmentation and its potential environmental risks in recirculating aquaculture system of Apostichopus japonicus

Abstract

A deep understanding of fecal characteristics and fragmentation mechanism is crucial for effectively removing the solid particles in recirculating aquaculture system (RAS) of Apostichopus japonicus, however, the underlying mechanism was still unclear. This study investigated the effects of diet with different sea mud addition on the fecal characteristics and microbiota. The experimental diet were the mixture of commercial feed and sea mud at the ratio of 1:1, 1:2, 1:3, 1:4 and 1:5, in which the added amount of sea mud was 50 %, 66.67 %, 75 %, 80 % and 83.33 %, respectively. The results showed that, at the end of aquaculture, Dx(50) in group 1:3 was the highest, while group 1:5 was the lowest. The fecal particle size distribution (PSD) appeared multi peak with the increase of sea mud addition. At the end of aquaculture, group 1:3 possessed the highest microbial diversity. BugBase phenotype prediction found that the microbial phenotypes in group 1:4 were mainly involved in functional expression, such as stress tolerance and potential pathogenicity. Significant correlation between environmental factors and fecal microbiota were found. Correlation analysis between fecal particle size and fecal microbiota showed that the abundance of pathogenic bacteria such as Vibrio, was higher on fecal particles with dispersed PSD. And NH4+-N removal rate decreased when Dx(10) decreased and Dx(90) increased. Thus, it was recommended that sea mud addition in diet should be <75 % in A. japonicus RAS.