Uncovering proteomics changes of Penicillium expansum spores in response to decanal treatment by iTRAQ

Abstract

Exogenous decanal can significantly inhibit the germination and development of postharvest pathogen Penicillium expansum in vitro. Through an iTRAQ-based analysis, a global view of proteomic alteration of P. expansum spores under decanal treatment was acquired. A total of 246 up-regulated and 293 down-regulated differentially expressed proteins (DEPs) were identified. Among them, DEPs related to glutathione metabolism, ribosome, and oxidative phosphorylation pathway were noticed for their functional significance, large number and high rich value in pathway enrichment statistics. Further analysis found that, under decanal stress, expression of 9 genes corresponding to DEPs involved in the oxidative phosphorylation pathway showed a significantly decreasing trend, and activities of 3 crucial enzymes (NADH dehydrogenase, CoQ-Cytochrome c reductase, and F(1)F(0)-ATP synthetase) were inhibited. Also, a decreased in ATP content, reduction in the number of mitochondria, and weakening in carbohydrate consumption were detected. Based on these results, disturbance of oxidative phosphorylation would partly be responsible for the inhibitory effect of decanal on the growth of P. expansum. The findings would provide new insights into exploring the possible antifungal mechanisms of decanal.