Unconventional Therapy with IgY in a Psoriatic Mouse Model Targeting Gut Microbiome.

Mihaela Surcel,Adriana Munteanu,Carolina Constantin,Alef Ibram,Gheorghita Isvoranu,Constantin Caruntu,Monica Neagu

doi:10.3390/jpm11090841

Abstract

Psoriasis has a multifactorial pathogenesis and recently it was shown that alterations in the skin and intestinal microbiome are involved in the pathogenesis of psoriasis. Therefore, microbiome restoration becomes a promising preventive/therapy strategy in psoriasis. In our pre-clinical study design using a mice model of induced psoriatic dermatitis (Ps) we have tested the proof-of-concept that IgY raised against pathological human bacteria resistant to antibiotics can alleviate psoriatic lesions and restore deregulated immune cell parameters. Besides clinical evaluation of the mice and histology of the developed psoriatic lesions, cellular immune parameters were monitored. Immune cells populations/subpopulations from peripheral blood and spleen cell suspensions that follow the clinical improvement were assessed using flow cytometry. We have quantified T lymphocytes (CD3ε+) with T-helper (CD4+CD8−) and T-suppressor/cytotoxic (CD8a+CD4−) subsets, B lymphocytes (CD3ε−CD19+) and NK cells (CD3ε−NK1.1+). Improved clinical evolution of the induced Ps along with the restoration of immune cells parameters were obtained when orally IgY was administered. We pin-point that IgY specific compound can be used as a possible pre-biotic-like alternative adjuvant in psoriasis.

Highlights

Psoriasis (Ps) is a complex and heterogeneous disease that affects the skin of the patients but echoes on several other organs [1]
We found the same tendency of variation for activation markers in spleen cell suspensions (Figure 10): significantly increased values for CD69, CD11c and CD28 in psoriatic dermatitis (Ps) group (p = 4.1 × 10−12; p = 2.1 × 10−7; p = 0.0001) compared to controls; the expression of NKp46 on NK1.1+ cells is lower in Ps mice as compared to controls, and the differences between the experimental groups were statistically significant (p = 0.0003)
Ps affecting the health of numerous individuals worldwide has a multifactorial pathogenesis and recently it was shown that alterations in the skin and intestinal microbiome are involved in the pathogenesis of Ps, microbiome restoration becomes a promising preventive/therapy strategy in this pathology

Summary

Introduction

Psoriasis (Ps) is a complex and heterogeneous disease that affects the skin of the patients but echoes on several other organs [1]. Recognized as a chronic autoimmune inflammatory disease, mediated mainly by T cells, Ps has important systemic manifestation [2], being frequently associated with psychological, metabolic, arthritic and cardiovascular comorbidities. Ps associated pathologies can lead to increased mortality and alters the clinical management of the patients. Prevalence of Ps varies depending on age, sex, geography, ethnicity, genetic and environmental factors [3]. It can occur at any age, the most common cases are reported before the age of 35, an age range that affects highly active individuals [4]

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