Ultrastructural studies of the CNS of TNAP-knock out mice

Abstract

Event Abstract Back to Event Ultrastructural studies of the CNS of TNAP-knock out mice Janos Barna1*, J. Hanics1, J. Xiao2, C. Fonta2 and L. Negyessy3 1 Department of Anatomy, Histology and Embryology, Semmelweis University, Hungary 2 Centre de Recherche Cerveau & Cognition, UMR CNRS 5549, France 3 Pázmány Péter Catholic University, Hungarian Academy of Sciences, Semmelweis University, Hungary Tissue-Nonspecific Alkaline Phosphatase (TNAP) is highly expressed in the node of Ranvier and the synaptic cleft mainly in the cerebral cortex of diverse species. It is assumed that TNAP plays a role in myelinization and the propagation of neuronal activity. Moreover, epileptic seizures are frequently observed in the serious form of hypophosphatasia caused by the mutation of the TNAP gene. Accordingly, TNAP-knock out (KO) mice, which die in about two weeks after birth, also suffers from epileptic seizures. The goal of our studies was to investigate the ultrastructural organization of the CNS, which is relevant to myelinization and synaptic transmission, with the aid of gene deletion (TNAP-KO) and electron microscopy (EM) in mice at postnatal days PND2, 7 and 8. Myelinization was studied in the spinal cord where it is more advanced as compared to the brain at the early postnatal days. We found that overall density of fibers are similar in the TNAP-KO and wild type (WT) mice by the end of the first postnatal week. However, KO mice have a larger proportion of partially myelinated axonal fibers and a reduced diameter of the fully myelinated axons as compared to that of the WT. Our preliminary data also show a significantly reduced number of premature and well developed synaptic junctions following gene deletion. These ultrastructural observations point on the possibility of an altered cortical excitability and decreased efficacy of activity propagation in the TNAP-KO mice. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Research on the cerebral cortex and related structures Citation: Barna J, Hanics J, Xiao J, Fonta C and Negyessy L (2009). Ultrastructural studies of the CNS of TNAP-knock out mice. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.188 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 06 Mar 2009; Published Online: 06 Mar 2009. * Correspondence: Janos Barna, Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary, barnaj@ana.sote.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Janos Barna J. Hanics J. Xiao C. Fonta L. Negyessy Google Janos Barna J. Hanics J. Xiao C. Fonta L. Negyessy Google Scholar Janos Barna J. Hanics J. Xiao C. Fonta L. Negyessy PubMed Janos Barna J. Hanics J. Xiao C. Fonta L. Negyessy Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.