Parathyroid hormone 2 receptor and its endogenous ligand TIP39 in the brain of macaque and human

Abstract

Event Abstract Back to Event Parathyroid hormone 2 receptor and its endogenous ligand TIP39 in the brain of macaque and human Attila G. Bago1, 2, Eugene Dimitrov3, Richard Saunders3, Laszlo Seress4, Miklos Palkovits2, Ted B. Usdin3 and Arpad Dobolyi2* 1 National Institute of Neurosurgery, Hungary 2 Hungarian Academy of Sciences and Semmelweis University, Department of Anatomy, Hungary 3 National Institute of Mental Health, United States 4 University of Pécs, Hungary Parathyroid hormone 2 receptor (PTH2R) and its ligand, tuberoinfundibular peptide of 39 residues (TIP39) form a neuromodulator system in rodents present in limbic, endocrine, auditory, and viscerosensory brain areas. In this study, we describe the presence and distribution of PTH2R and TIP39 in the primate brain. RT-PCR experiments revealed PTH2R mRNA expression in several areas and cell groups of the human brain. Subsequent in situ hybridization histochemistry in macaque brain confirmed high levels of PTH2R expression in the central amygdaloid nucleus, the medial preoptic area, the hypothalamic paraventricular and periventricular nuclei, the medial nucleus of the medial geniculate body and the pontine tegmentum. Fluorescent amplification immunochemistry studies in humans demonstrated high density of PTH2R-immunoreactive fibers in many brain regions including the medial preoptic area, the paraventricular hypothalamic nucleus, the median eminence, the paraventricular nucleus of thalamus, the periaqueductal gray, the parabrachial nuclei, the nucleus of the solitary tract, the spinal trigeminal nucleus, the medullary dorsal reticular nucleus, and the dorsal horn of the spinal cord. However, no immunolabeled cell bodies were found suggesting that PTH2R is promptly transported from cell bodies towards axon terminals. In contrast to the widespread distribution of PTH2R, TIP39 mRNA was detectable only in the subparafascicular area of the posterior thalamus and the medial paralemniscal nucleus in the lateral pons. The results demonstrate that TIP39 and the PTH2R are expressed in the brain of primates with locations suggesting involvement in regulation of fear, anxiety, reproductive behaviors, release of pituitary hormones, and nociception. Support was provided by the Hungarian Science Foundation NKTH-OTKA K67646 grant for AD, the consortial FP6 EU Grant BrainNet II LSHM-CT-2004-503039 for MP, and the NIMH Intramural Research Program for TBU. Arpád Dobolyi is grantee of the Bolyai János Scholarship. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Homeostatic regulatory mechanisms Citation: Bago AG, Dimitrov E, Saunders R, Seress L, Palkovits M, Usdin TB and Dobolyi A (2009). Parathyroid hormone 2 receptor and its endogenous ligand TIP39 in the brain of macaque and human. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.001 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Mar 2009; Published Online: 02 Mar 2009. * Correspondence: Arpad Dobolyi, Hungarian Academy of Sciences and Semmelweis University, Department of Anatomy, Budapest, Hungary, dobolyi@ana.sote.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Attila G Bago Eugene Dimitrov Richard Saunders Laszlo Seress Miklos Palkovits Ted B Usdin Arpad Dobolyi Google Attila G Bago Eugene Dimitrov Richard Saunders Laszlo Seress Miklos Palkovits Ted B Usdin Arpad Dobolyi Google Scholar Attila G Bago Eugene Dimitrov Richard Saunders Laszlo Seress Miklos Palkovits Ted B Usdin Arpad Dobolyi PubMed Attila G Bago Eugene Dimitrov Richard Saunders Laszlo Seress Miklos Palkovits Ted B Usdin Arpad Dobolyi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.