Abstract
Scanning and transmission electron microscopy studies of mitotic chromosomes have demonstrated that these chromosomes are composed of single intricately coiled and folded chromatin fibers 200-300 A in diameter (1). Transmission electron microscopy studies (2) have shown similar fibers in dipteran polytene chromosomes. It has been proposed that these fibers are extended in the less densely appearing interband regions and become more tightly coiled or folded at specific sites to produce the densely appearing banded regions (3). With the scanning electron microscope it is now possible to observe the characteristic appearance of the chromatin fibers in the band and interband regions of isolated intact polytene chromosomes. Furthermore, the morphology of the nucleolus and Balbiani rings can be observed as specialized structures in the chromosome.
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