Abstract
The small dark so-called B-neurons of She lumbar spinal ganglia of the frog (Rana temporaria) were investigated with the electron microscope as to the occurrence of glycogen. The animals were sacrificed during winter. At the light microscopic level the presence of glycogen was demonstrated with the periodic acid-Schiff reaction combined with diastase incubation. Spheroidal particles, 200–400 Å in size, here identified as glycogen, show a low electron density after uranyl acetate treatment, whereas after lead staining they appear opaque and stippled. In the perikaryon the glycogen particles were found both scattered and accumulated in dense aggregations. These particle aggregates often appeared in close morphological relation to a system of smooth-surfaced cytomembranes. Glycogen particles were also found inside some unmyelinated axons. Particles, ultrastructurally identical with the glycogen particles, were observed inside intramitochondrial vacuoles. This observation might suggest that, in this tissue, the mitochondria are directly involved in glycogen metabolism.
Published Version
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