Ultrasonic method for the whole blood coagulation analysis

Abstract

The developed ultrasonic method is based on the experimentally established fact that the ultrasound velocity in a blood sample is changing in a specific way during the blood clotting process. For ultrasound velocity measurements pulse echo method was selected. Implementation of this approach has a few problems caused by small dimensions of the measurement cell. All elements of the cell contacting with the blood are made of biologically compatible materials. The length of Pd coated chamber is 5 mm, volume 0.2 ml. Data of measurements are stored and processed by PC. The ultrasound velocity in a sample is displayed as it changes in time during the experiment; the temperature is monitored as well. Application of the digital filtering allows to smooth the coagulation curve and reach the sensitivity up to ±3 cm/s. The coagulation curves were obtained at frequency 5 MHz and they represent peculiar stages of blood clotting characterised by their duration and ultrasound velocity differences. Fine structure of ultrasonic velocity changes is registered from the very beginning of the clotting to lysis. Such experiments were carried out using blood samples taken from a few hundred volunteers. For dynamic calibration and periodic checking of the measuring system the liquid medium in which acrylamide polymerisation reaction takes place is proposed as a reference liquid. Such a liquid mimics clotting blood from the point of view of ultrasonic velocity changes.