Abstract
Encapsulation of physicochemical indicators and incorporation of FRET probes within model membrane vesicles has enabled valuable insights into membrane interactions, including pore formation, solution exchange, changes to membrane morphology and fusion dynamics. To characterize and quantify membrane interactions induced by a wide-variety of disruptive agents, including detergents and protein aggregates linked with neurodegenerative diseases, we have implemented high throughput fluorescence assays based on measuring the extent of Ca2+ entry into, and lipid mixing within, single surface-tethered vesicles.
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