Abstract
A very quick and easy LC-MS/MS analysis method for 5-HIAA (5-hydoxyindoleacetic acid) has been developed. The method was fully validated and proved to work well in a clinical setting. Precision at the upper reference limit 123 nmol/L was 3,3% CV. Accuracy ranged from 96% at low levels (50–100 nmol/L) to 99.7% at high levels (500 nmol/L). A previously reported reference interval of 35–123 nmol/L was confirmed as valid based on analysis of 40 samples from voluntary blood donors.
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