Abstract
An ultra-sensitive HPLC method for the determination of phenols in water based on flow-through reaction is described. The phenols are concentrated by a two-step liquid-liquid extraction with dichloromethane and after evaporation of the organic solvent the separation is carried out on an octadecyl-silica column by a gradient of water-acetonitrile. Detection is performed by an on-line twin detection system. First dinitrophenols are detected by UV-absorption measurement followed by oxidation of phenols with cerium(IV) in a tubular flow-through reactor and fluorescence measurement of cerium(III). Detection limits in the lower ppt range can be achieved. The influence of reaction time, temperature and reagent concentration on reaction yield was investigated. Identification and quantitation is improved by use of internal standards. The method was applied to samples of surface and drinking water.
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