UJI VIABILITAS POLEN PADA DUA SPESIES BELIMBING HUTAN ( Averrhoa dolichocarpa dan A. leucopetala )

Abstract

Wild starfruit Averrhoa dolichocarpa and A. leucopetala are new species reported. The aims of the research were to testing pollen viability at a day before anthesis (H -1 ) and at anthesis (H 0 ) stadia with germination in Brewbaker and Kwack (BK) and 10% sucrose media and staining test with 1% anilin blue and 1% I 2 KI. The optimum time for germination was 16 hours. Pollen viability in staining test results were higher than in germination test ie. 64.80% in BK medium, 54.60% in sukrosa, 88.60% in aniline blue dye and 88.50% in I 2 KI. BK medium showed a better result for germination test than 10% sucrose. In vitro viability germination test obtained the result that pollen viability value of wild starfruit at H 0 stadia was higher than pollen viability at H -1 stadia. While the result of staining test obtained that H -1 stadia was higher than at H 0 stadia. Long filament of Averrhoa dolichocarpa located on the slope had higher pollen viability than short filament. Filament factor in staining test was not significant. Pollen viability both in 10% sucrose to 1% aniline dye and 10% sucrose to 1% I 2 KI were negatively correlated. The correlation of pollen viability was not found either in BK medium and aniline dye blue or in BK medium and I 2 KI.