Abstract
Plant soursop (Annona muricata L.) is one of the Annonaceae familia. This plant has well-known in the society environment and it has been using as a traditional medicine. One of benefits of the soursop is can used as an algernative of cancer treatment. Until now not yet known scientifically whether soursop leaves have anticancer activity.This study aims to determine the potential toxicity of bioactive substances from the ethanol extract, of ethyl acetate and n-hexane of the soursop leaves as an anticancer agent by using Brine Shrimp Lethality Test (BSLT) method. This experimental As many as 100 larvas were use as research objects which divided into 3 groups. Each group consist of 10 larvas and the group repetition were done by randomly, three times per group. The extract concentration used in medium is 500, 100, 10 and as a control 0 ppm. Data obtained from counting the number of dead larvas (letal concentration 50 ppm) after 24 hours treatment. Based on the data obtained from LC50 of extract ethanol, ethyl acetate and n-hexane soursop leaves then determined by using probit analysis. The water content which contained in simplicia (drifed leaves) is 6,49%. The probit analysis results show that the ethanol extract have LC50 values of 86,45 ppm, 92,81 ppm of ethyl acetate extract and n-hexane extracts 37601,05 ppm. The distribution of ethanol and ethyl acetate extracts of soursop leaves in the experiments shown any potential toxicity against Artemia salina Leach, while the n-hexane extract of soursop leaves show no any potential toxicity to against Artemia salina Leach larvas.
Highlights
Plant soursop (Annona muricata L.) is one of the Annonaceae familia
Menurut Meyer et al, melaporkan bahwa dilihat persentase kematian larva dari ekstrak suatu ekstrakmenunjukkan daun sirsak pada konsentrasi 10 ppm yang aktivitasantikankerdalam ujitoksisitas jika paling tinggi adalah pada ekstrak etanol daun ekstrak dapat menyebabkankematian 50%
Penuntun Cara Modern MenganalisisTumbuhan. Terbitan kedua. Terjemahan K. Padmawinata dan l. Soediro. Bandung: ITB. [8] Carballo JL, Hernandez-Inda ZL, Perez P. Garcia-Gravaloz MD. 2002. Comparison between two brine shrimp assays to detec in vitro cytotoxicity in marine natural products. BMC Biotechnology. 2002;2: 1472-6570 [9] Anderson, J. E., 1991. A Blind Comparison of Simple Bench-Top Bioassays and Human Tumour Cell Cytotoxicities as Antitumor Prescreens. Phytochem. J Anal (2)
Summary
ABSTRAK Tanaman sirsak (Annona muricata L.) termasuk dalam familia Annonaceae.Masyarakat telah lama mengenal dan menggunakannya sebagai obat tradisional.Salah satu manfaat dari daun sirsak yaitu dapat dipakai sebagai alternatif pengobatan penyakit kanker.Sampai saat ini belum diketahui secara ilmiah apakah daun sirsak mempunyai aktivitas antikanker. Tujuan penelitian ini adalah untuk mengetahui potensi toksisitas zat bioaktif dari ekstrak etanol, etil asetat dan n-heksan daun sirsak sebagai zat antikanker dengan menggunakan metode BSLT.Penelitian ini menggunakan sebanyak 100 ekor larva udang yang digunakan sebagai objek penelitian yang secara acak dibagi dalam 3 kelompok.Tiap kelompok terdiri dari 10 ekor larva dan pengulangan dilakukan sebanyak tiga kali perkelompok.Konsentrasi ekstrak dalam media yang digunakan adalah 500, 100, 10 dan sebagai kontrol digunakan 0 ppm. Data yang diperoleh kemudian ditentukan dengan menggunakan tabel analisis probit. Hasil dari analisis probit menunjukkan bahwa ekstrak etanol mempunyai nilai LC50 sebesar 86,45 ppm, sedangkan ekstrak etil asetat 92,81 ppm dan ekstrak n-heksana 37601,05 ppm. Kata kunci :Daun sirsak, kanker, larva udang, Artemia salina Leach, Brine Shrimp Lethality Test, toksisitas
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