ANTIBACTERIAL ACTIVITIES TEST AND BRINE SHRIMP LETHALITY TEST OF Simargaolgaol (Aglaonema modestum Schott ex Engl.) LEAVES FROM NORTH SUMATERA, INDONESIA

Abstract

The research-based on empirical information from the community that used the leaves of Simargaolgaol (Aglaonema modestum Schott ex Engl.) as traditional treatments. This study aims to determine the class of phytochemical compounds contained in the extracted leaves samples based on the polarity of the solvent. Screening of phytochemical compounds was carried out using standard methods. Toxicity testing was carried out using the Brine Shrimp Lethality Test (BSLT) method for observing the mortality of A. salina Leach larvae and testing for biological activity as antibacterial conducted using the paper disc diffusion method. The study showed that the n-hexane extract had a class of steroid compounds; ethyl acetate extract contains alkaloids, flavonoids, saponins, and steroids while the ethanol extract contains alkaloids, flavonoids, saponins, and tannins. LC50 for n-hexane, ethyl acetate, and ethanol extract was 780.297, 33.083, and 20.548 respectively. The n-hexane and ethyl acetate extract had the moderate inhibitory ability on S. aureus, S. mutans, B. cereus, and S. viridans. The ethanol extract had strong activity as an antibacterial against S. mutans and B. cereus, and showed potential toxicity (LC50) to be developed as natural antibacterial drugs.