UJI KETAHANAN TIGA VARIETAS PISANG (Musa sp.) TERHADAP ISOLAST BANANA BUNCHY TOP VIRUS (BBTV)

Abstract

Banana is a horticultural crop that has economic value and is widely cultivated in tropical countries. Banana Bunchy Top Virus (BBTV) is the cause of dwarf disease in banana plants (Musa. sp). The vector for the spread of this banana dwarf disease is the banana aphid (Pentalonia nigronervosa). BBTV has been reported in various regions of Indonesia, having been reported in Bengkulu in 2020. However, data on the resistance of BBTV infections in Indonesia are still very limited. This study aimed to evaluate the resistance of three types of bananas (Barangan, Kepok, and Ambon Hijau) to BBTV infection. In this study, two treatments were carried out, namely inoculated and without BBTV virus inoculation with three types of bananas. BBTV detection was carried out using the PCR method. BBTV transmission using aphids succeeded in infecting the test plants with a disease incidence of 12.5% ​​to 75.0% at 30 DAI. The most common symptom seen is, slightly yellowed leaf edges. the severity of BBTV on the Barangan banana plant was 20.83%, kepok was 4.17% and Ambon Hijau was 25.00%. Samples on the leaves of the test banana plant that showed symptoms of BBTV were taken for detection using Polymerase Chain Reaction (PCR) with a pair of related NSP primers in the BBTV genome with a size of ± 500 bp. The results of the detection of BBTV virus showed that the types of bananas that were resistant to BBTV were Kepok bananas, Barangan bananas and Ambon Hijau types which showed moderate resistance to BBTV