Abstract

Oil palm empty fruit bunches (OPEFB) is one of the largest types of solid waste produced by palm oil mills (POM). Each processing of 1 ton of fresh fruit bunches (FFB) produces 220–230 kg of empty fruit bunches (EFB). One of the attempts to overcome the accumulation of EFB is by composting. The main obstacle in composting OPEFB is the process of overhauling, it takes 6-12 months because OPEFB contains 33.02% cellulose, 22.05% hemicellulose and 35.08% lignin. One of the efforts to speed up the composting is using biological treatment by adding microorganisms such as cellulolytic bacteria which are capable in producing cellulase enzymes. The reseach was carried out experimentally using a non-factorial completely randomized design (CRD) consisting of eight treatments, namely B0 (control), B1
 
 (Bacillus tequilensis Strain RA 1402), B2 (Bacillus subtilis Strain C17), B3 (Bacillus subtilis Strain SKUASIS) , B4 (Bacillus subtilis Strain DSM 10), B5 (Bacillus subtilis Strain K43), B6 (Pseudomonas aeruginosa Strain KUJM) and B7 (Combined Isolates). The parameters observed were physical properties of compost, weight loss of compost and total of compost microb population. The resultof the research showed that cellulolytic bacteria isolates had a significant effect on weight loss of compost, pH. The combined isolates (B7) is the best treatment in increasing compost material weight, pH. The treatment B7 has the best ability in producing high quality compost which is under the SNI Compost Quality Standard: 19?7030?2004.
 
 
 Keyword: Oil palm empty bunches, cellulolytic bacteria, composting.

Highlights

  • of the largest types of solid waste produced by palm oil mills

  • of the attempts to overcome the accumulation of empty fruit bunches (EFB) is by composting

  • of the efforts to speed up the composting is using biological treatment

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Summary

BAHAN DAN METODE Peremajaan Sumber Isolat Bakteri Selulolitik

Peremajaan isolat bakteri selulolitik dilakukan dengan menginokulasikan satu ose isolat bakteri selulolitik ke dalam media CMC padat dengan menggunakan metode gores (Streak Plate). Pembuatan Starter Bakteri Selulolitik Satu ose isolat bakteri selulolitik hasil peremajaan diinokulasikan pada 3 ml media CMC cair, lalu diinkubasi selama 1 × 24 jam, setelah itu hasil isolat tersebut diinokulasikan pada 50 ml media CMC cair dan diinkubasi selama 3 × 24 jam menggunakan shaker dengan kecepatan 120 rpm (starter), selanjutnya 50 ml tersebut ditambahkan akuades 300 ml kemudian dituang ke dalam botol semprot dan diaplikasikan ke bahan kompos (Gusmawartati, 1999). Pengamatan Pengambilan sampel kompos dilakukan secara manual dengan menggunakan tangan yang telah memakai sarung tangan. Pengamatan penyusutan berat bahan kompos (%) dilakukan dengan menimbang berat bahan awal kompos dan berat bahan akhir kompos setelah minggu ke−8 dengan menggunakan timbangan. Suspensi tanah diukur dengan pH meter yang telah dikalibrasi menggunakan larutan buffer pH 4,0 dan pH 7,0, pengukuran dilakukan pada minggu ke−8

Rancangan Penelitian dan Analisis Data Penelitian ini menggunakan Rancangan
HASIL DAN PEMBAHASAN Sifat Fisik Kompos
Hitam Hitam
Gabungan isolat
Bacillus tequilensis Strain
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