Abstract
Infectious diseases are one of the main causes of diseases in tropical regions like Indonesia. Infectious diseases usually can be cured by antibiotics, since the use of antibiotics has side effects and long-term resistance and prices that are not affordable, it needs a role of herbs that can weaken the activity of these bacteria. Kersen plants contains various chemical compounds namely flavonoid compounds, saponins, and tannins that have antioxidant potential and antibacterial activity. Belimbing wuluh contains flavonoid compounds. This research was conducted to find out the antibacterial activity of extract combination of kersen leaf (Muntingia calabura L) and belimbing wuluh leaf (Averrhoa bilimbi L) against Staphylococcus aureus. Extract of Kersen leaf and Belimbing wuluh leaf were obtained by maceration method using ethanol 96% for 5 days. The antibacterial activity test was performed by diffusion method using disc paper based on the inhibit zone diameter or clear zone formed around the disc paper. The test was conducted by extract comparison of kersen leaf and belimbing wuluh leaf at 30% concentration namely 1:1, 1:2, 2:1, 1:3 and 3:1 against Staphylococcus aureus. The results showed that the extract comparison of kersen leaf and belimbing wuluh leaf 1:1 had a weak inhibitory power because of its inhibitory value ≤ 5 mm, that was 4.67 mm. While the ratio of 1:2, 2:1, 1:3 and 3:1 had a moderate inhibition resistance value ranges from 5-10 mm; 6.33 mm, 7.16 mm, 7.67 mm, and 7.83 mm. Extract rendemen of kersen leaf was 11.94% and belimbing wuluh leaf was 6.28%.
Highlights
Infectious diseases are one of the main causes of diseases in tropical regions like Indonesia
The results showed that the extract comparison of kersen leaf and belimbing wuluh leaf 1:1 had a weak inhibitory power because of its inhibitory value ≤ 5 mm, that was 4.67 mm
Khasiat Belimbing Wuluh ( Averrhoa bilimbi L .) terhadap Penyembuhan Acne Vulgaris
Summary
Alat yang digunakan cawan petri, kertas cakram, pipet volume, oven, inkubator, autoklaf, timbangan analitik, penggaris, waterbath, alat-alat gelas, jarum ose, batang. Bahan yang digunakan daun kersen dan belimbing wuluh, bakteri Staphylococcus tinggi dapat menghambat dengan baik bakteri Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus dan Bacillus subtilis [3]. Kloroform dan metanol daun kersen dapat menghambat bakteri Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans dan Microsporum canis [5]. Menurut Pendit et al, daun belimbing wuluh yang diekstraksi menggunaka pelarut etanol dapat menghambat aktivitas bakteri Staphylococcus aureus sebesar 13,13 mm aureus, etanol 96%, akuades, Nutrient Agar, HCl pekat, serbuk Mg, FeCl3 1%, NaOH 1 N, asam sulfat encer P, iodium 0,1 N, HCl 2 N, pereaksi Mayer, etil asetat, n-heksana, asam asetat , n-butanol, kloroform, dan metanol
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