Çuha Çiçeği (Oenothera biennis) ve Tatlı Badem (Prunus dulcis Mill) Yağlarının Anti-enzim Aktivitelerinin Araştırılması

Abstract

In our study, the question of what might be the effects of Primrose (Oenothera biennis) and sweet almond (Prunus dulcis Mill.) oils on two different enzyme activities was asked. The first enzyme in our study is carbonic anhydrase I-II isoenzymes that play a very important role in many physiological events, by transforming catalysis of CO2 hydration and HCO3-dehydration in living things, by creating an intracellular bicarbonate buffer system. The second enzyme, the acetylcholinesterase enzyme, which was located in the synapse between the muscle cell and nerve, catalyzes the decomposition of the acetylcholine molecule. Inhibitors of both enzymes have the potential to be drugs. Therefore, the inhibition effect of the specified oils was investigated. For this purpose, the first carbonic anhydrase I-II isoenzyme was purified by the sepharose-4B-L tyrosine-sulfonamide affinity column. Then, by looking at enzyme activity in at least five different inhibitor concentrations, effects of evening primrose (Oenothera biennis) and sweet almond (Prunus dulcis Mill.) oils on enzyme activities were investigated. Finally, IC50 values of Primrose (Oenothera biennis) and sweet almond (Prunus dulcis Mill.) oils were determined by drawing activity%-[I] graph. Carbonic anhydrase I isoenzyme was purified from human erythrocytes 119 times in 20.12% yield, while carbonic anhydrase II isoenzyme was purified 535.72 times in 83.05% yield. IC50 values of the primrose oils (Oenothera biennis) on carbonic anhydrase I-II and acetylcholinesterase enzyme activity were 0,1950, 0,1406 and 0,1097 mg / mL, respectively. IC50 values of the sweet almond oil (Prunus dulcis Mill.) on carbonic anhydrase I-II and acetylcholine esterase enzyme activity were 0.0345, 0.0266 and 0.0394 mg/mL respectively. When both oils used in the study are compared, we see that sweet almond oil (Prunus dulcis Mill.) is more effective on both enzyme activities. Therefore, instead of synthetic drugs, sweet almond (Prunus dulcis Mill.) oil is thought may be used as a supplement in the treatment of diseases caused by the enzymes in question.