TYROSINE KINASE PROTEINS TKS4 AND TKS5 PARTICIPATE IN THE INVASIVENESS OF SALIVARY GLAND MUCOEPIDERMOID CARCINOMA

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Objectives The aim of this study was to verify the expression of Tks4 and Tks5 and the role played by these proteins in the invasiveness of human salivary mucoepidermoid carcinoma (HMC). Study Design Immunofluorescence was performed in HMC cells. Tks4 and Tks5 genes were silenced by short hairpin RNA (shRNA). Western blot and polymerase chain reaction were performed to confirm silencing of Tks4 and Tks5 in gene and protein expression levels. After silencing, 3 cell lines were obtained: HMC (Tks5-), HMC (Tks4-), and HMC-CT (control), and cell viability assays were performed. Cell invasion assays were performed by comparing silenced and nonsilenced cell lines. Mann-Whitney statistical test was performed to compare the cell lines. Results HMC-CT expresses Tks4 and Tks5. There was inhibition of Tks4 and Tks5 at gene and protein levels. Viable and stable cell lines were obtained after silencing. In the cell invasion assay, a greater invasion of cells was observed in the nonsilenced cell line (HMC-CT) when compared to the silenced cell lines (P < .001***). Conclusions Knockdown of Tks4 and Tks5 reduce invasiveness in HMC cell lines. This is the first in vitro evidence that shows the possible key role of Tks4 and Tks5 in the invasion of HMC. The aim of this study was to verify the expression of Tks4 and Tks5 and the role played by these proteins in the invasiveness of human salivary mucoepidermoid carcinoma (HMC). Immunofluorescence was performed in HMC cells. Tks4 and Tks5 genes were silenced by short hairpin RNA (shRNA). Western blot and polymerase chain reaction were performed to confirm silencing of Tks4 and Tks5 in gene and protein expression levels. After silencing, 3 cell lines were obtained: HMC (Tks5-), HMC (Tks4-), and HMC-CT (control), and cell viability assays were performed. Cell invasion assays were performed by comparing silenced and nonsilenced cell lines. Mann-Whitney statistical test was performed to compare the cell lines. HMC-CT expresses Tks4 and Tks5. There was inhibition of Tks4 and Tks5 at gene and protein levels. Viable and stable cell lines were obtained after silencing. In the cell invasion assay, a greater invasion of cells was observed in the nonsilenced cell line (HMC-CT) when compared to the silenced cell lines (P < .001***). Knockdown of Tks4 and Tks5 reduce invasiveness in HMC cell lines. This is the first in vitro evidence that shows the possible key role of Tks4 and Tks5 in the invasion of HMC.