Abstract
1. 1. Tyrosinase was purified from melanosomal fraction of hamster melanoma. 2. 2. A radioimmunoassay was developed to quantitate the tyrosinase protein in hamster serum and hamster melanoma tissue using polyclonal anti-tyrosinase antibodies and 125I-labeled enzyme. 3. 3. The serum tyrosinase levels were found to be about 0.24 μg and 1.14–4.48 μg/ml in normal hamsters and melanoma-bearing hamsters, respectively. 4. 4. Tyrosinase protein in serum correlated significantly with the enzyme activity in hamsters with melanoma ( r = 0.733). 5. 5. In the cytosol fraction of hamster melanoma, a level of 2.2 μg of tyrosinase/mg protein was determined. 6. 6. The usefulness and possible applications of the tyrosinase radioimmunoassay are discussed.
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