Abstract
PCR was conducted on tissues of pigs suspected to suffer from PCV2-associated diseases, and differentiation PCR for PCV1 and PCV2 was conducted on PCV positive samples. The results showed that 117 out of the 192 tissue samples were PCV positive, all of which harbored PCV2 genome, two of which harbored PCV1 simultaneously. PCV2 were cloned from the samples only harbored PCV2, and the sequences obtained all belonged to PCV2–1. Infectious clone viruses were constructed using two of these sequences, and the rescued viruses were named PCV2(2–1) and PCV2(4–1). Cultivation of these viruses showed that they replicated efficiently in cells maintained by alteration of culture medium.
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