Abstract
Objectives Enhanced migration and osteogenic differentiation of mesenchymal stem cells (MSCs) are beneficial for MSC-mediated periodontal tissue regeneration, a promising method for periodontitis treatment. FBXO5, a member of the F-box protein family, is involved in the osteogenic differentiation of MSCs. Here, we investigated the effect of FBXO5 on human periodontal ligament stem cells (hPDLSCs). Materials and Methods hPDLSCs were isolated from periodontal ligament tissue. Lentivirus FBXO5 shRNA was used to silence FBXO5 expression. Two transcripts of FBXO5 were overexpressed and transduced into hPDLSCs via retroviral infection. Migration and osteogenic differentiation of hPDLSCs were evaluated using the scratch migration assay, alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, western blotting, and real-time polymerase chain reaction. Results The expression of FBXO5 was upregulated after osteogenic induction in hPDLSCs. FBXO5 knockdown attenuated migration, inhibited ALP activity and mineralization, and decreased RUNX2, OSX, and OCN expression, while the overexpression of two transcript isoforms significantly accelerated migration, enhanced ALP activity and mineralization, and increased RUNX2, OSX, and OCN expression in hPDLSCs. Conclusions Both isoforms of FBXO5 promoted the migration and osteogenic differentiation potential of hPDLSCs, which identified a potential target for improving periodontal tissue regeneration.
Highlights
Periodontitis, one of the most widespread oral chronic inflammatory diseases, is the main cause of adult tooth loss and may be associated with diseases such as diabetes and atherosclerotic cardiovascular diseases [1, 2]
The Expression of FBXO5 Was Upregulated in Human periodontal ligament stem cells (PDLSCs) (hPDLSCs) after Osteogenic Induction
We found that FBXO5 expression increased in hPDLSCs at 5, 7, and 14 days after osteogenic induction (Figures 1(a) and 1(b))
Summary
Periodontitis, one of the most widespread oral chronic inflammatory diseases, is the main cause of adult tooth loss and may be associated with diseases such as diabetes and atherosclerotic cardiovascular diseases [1, 2]. Mesenchymal stem cell- (MSC-) mediated periodontal tissue regeneration is regarded as a promising method for periodontitis treatment [4]. MSCs are multipotent cells with self-renewal capacity and multidirectional differentiation ability. The decreased number and impaired function of periodontal ligament stem cells (PDLSCs) in patients with periodontitis pose difficulty in achieving the desired tissue regeneration [9, 10]. The transplantation of autologous or allogeneic MSCs into periodontal lesion areas was shown to promote periodontal tissue regeneration. Human PDLSCs (hPDLSCs) possess superior abilities to differentiate into bone, cementum, and periodontal ligament and may serve as a potential source for tissue engineering [13,14,15,16]. In comparison with human bone marrow MSCs, hPDLSCs offer several advantages [17, 18]. HPDLSCs display higher proliferation rate and express specific transcription
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
