Two-, three-, and four-dimensional NMR methods for obtaining larger and more precise three-dimensional structures of proteins in solution.

Abstract

CONTENTS PE~S~’ECTIVES AND OVERVIEW 30 PRINCIPLES OF 3DaND 4D NM~ 31 S~Q~ ~SS~G~ ~S~G ~O~UC~ 3~ ~ 34 Assignment of Spin Systems 34 Conventional Sequential Assi9nment Usin9 3D NMR 42 Sequential Assi#nment Via Well-Resolved One-Bond J Couplings 42