Two Simple Spectrophotometric Methods for the Simultaneous Determination of Amoxicillin Trihydrates and Flucloxacillin Sodium

Abstract

Simple, specific, accurate and precise spectrophotometric methods were developed and validated for simultaneous estimation of amoxicillin Trihydrates and flucloxacillin Sodium in their pure form and pharmaceutical dosage form. Two spectrophotometric methods were applied: derivative ratio spectra and absorbance ratio method. The linearity range for the spectrophotometric methods was found to be( 1-40µg/mL) and( 0.7-40µg/mL) for amoxicillin and flucloxacillin respectively in derivative ratio method and to be (1.5-65µg/mL) for bothamoxicillin and flucloxacillin in absorbance ratio method. Both methods were simple, sensitive, ecofriendly and don’t need a special program, so they could be easily applied as alternative methods to LC methods in quality control laboratories lacking the required facilities for those techniques.