Abstract
A new kinetic-based method for simultaneous determination of binary mixtures is proposed that is simple, rapid, and minimizes errors arising from drift. This new method is based on the measurement of the rate at two points during the course of the enzymatic reaction and is therefore referred to as a two-rate method. The performance of the method is evaluated by the l-amino acid oxidase/Trinder reaction for the determination of binary mixtures of l-amino acids under the same conditions using air-segmented continuous flow/stopped-flow methodology. Some assumptions with this method are presented and supported by experimental data.
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