Abstract
Listeria ivanovii subsp. ivanovii is an intracellular bacterium distributed widely in nature, causing the listeriosis in ruminants and humans. Previous researches had isolated 116 strains of L. ivanovii subsp. ivanovii from wild rodents and pikas of different regions in China, and the predominant sequence types were ST1 and ST2. In this study, we first investigated the biological characteristics and virulence of these two clonal strains including motility, metabolism and virulence in cells and mouse model. The results demonstrated the ST1 strains exhibited motility, wide metabolic activity and hypervirulence, whereas the ST2 strains showed non-motility, relative lower metabolic activity and virulence. Considering the transmissible ability from wild rodents and pikas to ecological environment, the L. ivanovii subsp. ivanovii with potential pathogenicity to humans and ruminants should be monitored.
Highlights
Listeria ivanovii and Listeria monocytogenes, which are the only two pathogenic in the genus Listeria, appear to be widely distributed in a variety of environments such as food, water, soil and feces of humans and animals [1,2,3]
In ST2 strains, a premature stop codon (PMSC) was identified in gmaR, which was important in regulating expression of flagellar genes such as flaA, and the relative expression levels of gmaR and flaA in LIV047 were significantly lower than that in PAM55 and LIV037 (P < 0.01) using real-time PCR (Figure 2E)
Previous studies have noted that wild rodents and pikas in China carried various STs of L. ivanovii subsp. ivanovii, and the predominant sequence types were ST1 and ST2 [12, 13]
Summary
Listeria ivanovii and Listeria monocytogenes, which are the only two pathogenic in the genus Listeria, appear to be widely distributed in a variety of environments such as food, water, soil and feces of humans and animals [1,2,3]. L. monocytogenes can cause various clinical manifestations such as septicemia, meningoencephalitis and diarrhea in human and animals. L. ivanovii can cause ruminant infection with similar pathological manifestations except meningoencephalitis, which is one of the characters of L. monocytogenes infection in ruminants and humans [2, 4, 5]. Sporadic listeriosis caused by L. ivanovii in immunocompromised people have been reported [5, 6]. The two subspecies of L. ivanovii, which are subsp. Ivanovii caused the most of listeriosis in human and animals, Listeria ivanovii Strains in China and a previous study had found L. ivanovii subsp. Ivanovii clinical strain G770 contains a type I restriction-modification system which may contribute to its hypervirulence [1, 6] L. ivanovii subsp. ivanovii caused the most of listeriosis in human and animals, Listeria ivanovii Strains in China and a previous study had found L. ivanovii subsp. ivanovii clinical strain G770 contains a type I restriction-modification system which may contribute to its hypervirulence [1, 6]
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