Two new species of the industrially relevant genus Absidia (Mucorales) from soil of the Brazilian Atlantic Forest

Diogo Xavier Lima,Catarina Ferreira de Lima,Joana D’Arc Alves Leitão,Thuong Thuong Thi Nguyen,Luciana Melo Sartori Gurgel,Suzana Brito Gomes da Silva,Thalline Rafhaella Leite Cordeiro,André Luiz Cabral Monteiro de Azevedo Santiago,Hyang Burm Lee

doi:10.1590/0102-33062020abb0040

Diogo Xavier Lima, Catarina Ferreira de Lima + Show 7 more

Open Access

https://doi.org/10.1590/0102-33062020abb0040

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Abstract

During a survey of Mucorales in soil from an upland forest area in Pernambuco, Brazil, two specimens were isolated and characterized based on their morphological, physiological, and molecular data (ITS and LSU rDNA). Phylogenetic analyses of the isolates revealed that the strains URM 8209 and URM 8210 are closely related to species of Absidia. URM 8209 forms conical, subglobose, and strawberry-shaped columellae and the sporangiospores are cylindrical and ellipsoid. URM 8210 produces hemispheric, subglobose, and strawberry-shaped columellae and the sporangiospores are globose, subglobose, ellipsoid, and short cylindrical. Based on evidence obtained through analysis of datasets (LSU and ITS rDNA regions), A. saloaensis sp. nov. (URM 8209) and A. multispora sp. nov. (URM 8210) are proposed here as novel species of Absidia. A table with morphological characteristics of Neotropical Absidia spp. is provided.

Highlights

The genus Absidia is composed of cosmopolitan fungal species commonly isolated from soil, herbivorous dung and decaying substrates
Rhizoids are never opposed to sporangiophores (Benny 2001), and columellae may be conical, subglobose, or aplanate, frequently showing an apical projection
The phylogenetic relationship of two novel species and related species was determined by analysis of concatenated sequences datasets of two loci (ITS and large subunit (LSU)) (Fig.1)

Summary

Introduction

The genus Absidia is composed of cosmopolitan fungal species commonly isolated from soil, herbivorous dung and decaying substrates (van Tieghem 1878). Species of this genus commonly produce sporangiophores in whorls, arising from stolons that bear apophysate and pyriform sporangia with a deliquescent wall. Absidia glauca performs biotransformation of 3-Oxo-Oleanolic acid resulting in hydroxylated metabolites and both species mentioned above are excellent chitosan producers used in food processing, antimicrobial production and biotransformation of steroid products (Abdel-Fattah et al 1984; Smith et al 1989; Muzzarelli et al 1994; Brzezowska et al 1996; Huszcza & Gladysz 2003 Rungsardthong et al 2006; Dai et al 2009).

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