Abstract
Chromatographic fractionation of the organic extract obtained from the brown alga Cystoseira trinodis led to the isolation of a known carotene pigment (7), five C-29 steroids (2–6), including two new ones (2 and 5), and an unexpected metabolite, halogenated cyclic ene-yne ether (1). Compound 1 was isolated from the genus Cystoseira for the first time. This finding plays an important role in the chemo-taxonomical differentiation of C. trinodis from other Cystoseira species. All compounds (1–7) were evaluated for their in vitro cytotoxic activities against four human tumor cell lines MCF-7, HepG2, WI 38, and Vero. They showed moderate cytotoxicity with IC50 in the range 7–33 μM, where 7 exhibited the highest antioxidant activity with 72.1% inhibition.
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