Abstract
Two new C21 steroidal glycosides, paniculatumosides H and I, together with four known ones were isolated from the roots of Cynanchum paniculatum (Bge.) Kitag. Their structures were identified by spectroscopic methods including extensive 1D and 2D NMR techniques. All compounds were subjected to detect the anti-tobacco mosaic virus (TMV) activities and their cytotoxities against three human tumor cell lines (SMMC-7721, MDA-MB-231 and A549). The results showed that compounds 1 and 5 exhibited potent protective activities against TMV, while 2, 4 and 6 had moderate effects on the SMMC-7721 cancer cells viability.Graphical
Highlights
Cynanchum paniculatum (Bunge) Kitag is a vivacious herb broadly distributed in China, Japan and Korea, whose dried roots have been used as a Chinese herbal medicine for the
The 1H NMR spectrum of compound 1 showed the presence of two tertiary methyl groups at δH 0.92 (3H, s, H-19) and δH 1.53 (3H, s, H-21), one olefinic proton at δH 5.38 (d, J = 5.3 Hz, H-6), one olefinic deshielded proton at δH 6.24 (s, H-18), two oxygen-substituted methine protons at δH 3.44 (m, H-3) and δH 5.29 and two methylene protons at δH 3.84 (t, J = 8.6 Hz, H-15a) and δH 4.15
By comparison of its 1H and 13C NMR spectra (Table 1) to those of glaucogenin C (3) indicated that the aglycone of 1 is glaucogenin C, which confirmed by correlations of H-19/H-2b, H-19/H-8, H-1b/H-3, H-21/H-16 and H-16/H-17 in the ROESY experiment (Fig. 2)
Summary
Cynanchum paniculatum (Bunge) Kitag is a vivacious herb broadly distributed in China, Japan and Korea, whose dried roots have been used as a Chinese herbal medicine for the. Neocynapanogenin F 3-O-β-d-oleandropyranoside (6) [10] were isolated. We tested the anti-TMV activities and cytotoxicity of these compounds (Fig. 1)
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