Two Mutations in TP53 Promotes Migration, Invasion, and Proliferation of Human Ovarian Cancer A2780 Cells

Abstract

The ovarian cancer cell line A2780 without endogenous TP53 expression was selected. A plasmid carrying wild-type TP53, TP53 with two point mutations G199X and V157fs, or an empty vector was transfected in A2780 cells with lipofection. Western blot analysis was used to investigate whether the mutant plasmid affects the expression of P53 protein. In addition, the effect of mutation on the migration of A2780 cells was detected with scratch assay, while that on cell invasion was evaluated with transwell assay. We also studied the effect of mutation on the proliferation of A2780 cells with the cell counting kit-8 (CCK-8) assay. As a result, we found that the cells transfected with TP53 G199X and V157fs mutants and empty vector failed to express P53 protein. The migration ability of the cells expressing the two mutant genes was higher than that of the cells expressing the wild-type gene. The invasion ability of the two mutant groups was significantly higher than that of the wild-type group. Cell proliferation was also higher for the two mutant groups and the empty vector group than for the wild-type group. Thus, the two point mutations in TP53 (G199X and V157fs) gene could terminate the expression of P53 protein and promote migration, invasion, and proliferation of ovarian cancer A2780 cells.