Abstract
NAD-malic enzyme (EC 1.1.1.39) activity, expression and methylation of promoters of its two genes was studied in maize (Zea mays L.) leaves depending on light regime. The total activity was high in darkness and upon irradiation by far-red light and suppressed by white light and by red light. The changes in the levels of transcripts of the genes Me1 and Me2 encoding NAD-malic enzyme revealed their dependence on irradiation in opposite ways. White and red light decreased the quantity of mRNA of the gene Me1, while far-red light led to the increase of its transcripts. The opposite pattern was observed for the transcripts of Me2, the level of which was low in darkness and upon irradiation by far-red light, and was higher in white light and after irradiation by red light. The study of methylation of the promoters of the genes encoding NAD-ME showed a strong dependence between the levels of transcripts and the state of methylation of CG dinucleotides. The two isoforms of NAD-malic enzyme were partially purified from maize leaves and characterized. The first isoform had a pH optimum of 6.4 while the second had a pH optimum of 6.9; in the reverse reaction, the pH optimum was ∼0.5 units higher. It is concluded that the two genes encode different isoforms of NAD-malic enzyme with different kinetic properties. The role of both isoforms in the operation of the tricarboxylic acid cycle in the open mode is discussed.
Published Version
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