Two-dimensional iron porphyrin nanozyme mimics cytochrome P450 activity for cancer proliferation inhibition

Abstract

Two-dimensional materials have excellent performance in energy catalysis and biomedicine due to their unique advantages in surface and interfacial properties. Taking into account the unique advantages of the surface interface and catalysis of two-dimensional materials, it is a very feasible way to construct two-dimensional materials with clear atomic structure to break the redox balance in tumor cells, so as to achieve efficient anti-tumor effects. Based on this, we selected the porphyrin ligands that widely exist in nature, equipped with iron metal active centers, and constructed a two-dimensional iron porphyrin nanozyme with a clear atomic structure, which simulated the redox activity of cytochrome P450 enzymes. The experimental results show that by carrying a rotatable pyridine group on the porphyrin ring, the iron porphyrin monomers can be effectively connected and expanded into a two-dimensional iron porphyrin microstructure. Enough iron centers are exposed on the surface of two-dimensional iron porphyrins as catalytic sites. In the presence of oxidants, two-dimensional iron porphyrins can efficiently catalyze the oxidation of small organic molecules, and have good P450-like enzymatic activity. At the same time, the two-dimensional iron porphyrin nanomaterial was used for anti-tumor, and it was found that it has an efficient ability to inhibit tumor proliferation, which may be due to the catalytic oxidation of various organic small molecules in tumor cells by the high concentration of hydrogen peroxide in tumor cells. At the same time, we found that the two-dimensional iron porphyrin nanozyme may have a good drug loading capacity due to the many exposed iron sites on its surface, which provides a possible option for drug loading to achieve combined high-efficiency tumor therapy.